human OTUD5 shRNA silencing Adenovirus

Name 
Ad-h-OTUD5-shRNA
Cat No 
shADV-218004
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human OTUD5.

shADV-218004
Ad-h-OTUD5-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human OTUD5 (OTU deubiquitinase 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
deubiquinating enzyme A; deubiquitinating enzyme A; DUBA; OTU domain containing 5; OTU domain-containing protein 5
Description (eCommerce)
Deubiquitinating enzyme A (DUBA) is a negative regulator of the innate immune system. DUBA has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains and acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q96G74].
Description (Vector)
OTUD5 encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described.
Gene ID
55593
Gene Name (eCommerce)
OTU deubiquitinase 5
Gene Name (Vector)
OTU deubiquitinase 5
Gene Symbol
OTUD5
HGNC ID
HGNC:25402
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1629
ORF Size (bp)
1629 bp
Protein Name (eCommerce)
OTU domain-containing protein 5
RefSeq ID
NM_017602
RefSeq Synonyms
NM_017602, NM_001136159, NM_001136158, NM_001136157, BC098440, BC028225, BC009917,
Research Areas
Cancer,IFN Signaling,Immunology,Innate Immunity,Signal Transduction,Toll-like Receptor Signaling,Ubiquitination
Research Areas (Faceted)
cancer,cell_biology,immunology,signal_transduction
Species
human
Target Sentence
Deubiquitinating enzyme A (DUBA) is a negative regulator of the innate immune system. DUBA has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains and acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q96G74].
UniGene ID
Hs.496098
UniProt ID (eCommerce)
Q96G74

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.