human NT5E shRNA silencing Adenovirus

Name 
Ad-h-NT5E-shRNA
Cat No 
shADV-217149
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NT5E.

shADV-217149
Ad-h-NT5E-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NT5E (5′-nucleotidase ecto). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
5'-NT; 5'-nucleotidase; CALJA; CD antigen CD73; CD73; E5NT; ecto-5'-nucleotidase; eN; eNT; NT; NT5; NTE; Purine 5-Prime-Nucleotidase
Description (eCommerce)
CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. CD73 is used as a determinant of lymphocyte differentiation. [provided by RefSeq, Mar 2011] [taken from NCBI Entrez Gene (Gene ID: 4907)].
Description (Vector)
NT5E is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
4907
Gene Name (eCommerce)
5'-nucleotidase ecto
Gene Name (Vector)
5'-nucleotidase ecto
Gene Symbol
NT5E
HGNC ID
HGNC:8021
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1725
ORF Size (bp)
1725 bp
Protein Name (eCommerce)
5'-nucleotidase
RefSeq ID
NM_002526
RefSeq Synonyms
NM_002526, NM_001204813, BC065937, BC015940,
Research Areas
Immunology,Metabolism/Metabolic Process,Signal Transduction
Research Areas (Faceted)
cell_biology,immunology,metabolism,signal_transduction
Species
human
Target Sentence
CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. CD73 is used as a determinant of lymphocyte differentiation. [provided by RefSeq, Mar 2011] [taken from NCBI Entrez Gene (Gene ID: 4907)].
UniGene ID
Hs.153952
UniProt ID (eCommerce)
P21589

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.