human NLE1 shRNA silencing Adenovirus

Name 
Ad-h-NLE1-shRNA
Cat No 
shADV-216777
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NLE1.

shADV-216777
Ad-h-NLE1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NLE1 (notchless homolog 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
NLE; Notchless gene homolog; notchless protein homolog 1
Description (eCommerce)
Notchless protein homolog 1 (NLE1) plays a role in regulating Notch activity. It is involved in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. NLE1 is required during embryogenesis for inner mass cell survival (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NVX2].
Gene ID
54475
Gene Name (eCommerce)
notchless homolog 1
Gene Name (Vector)
notchless homolog 1
Gene Symbol
NLE1
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1458
ORF Size (bp)
1458 bp
Protein Name (eCommerce)
Notchless protein homolog 1
RefSeq ID
NM_018096
RefSeq Synonyms
NM_018096, NM_001014445, BC012075,
Research Areas
Notch Signaling,Signal Transduction
Research Areas (Faceted)
signal_transduction,developmental_biology
Species
human
Target Sentence
Notchless protein homolog 1 (NLE1) plays a role in regulating Notch activity. It is involved in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. NLE1 is required during embryogenesis for inner mass cell survival (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NVX2].
UniGene ID
Hs.85570
UniProt ID (eCommerce)
Q9NVX2

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.