human NEK1 shRNA silencing Adenovirus

Name 
Ad-h-NEK1-shRNA
Cat No 
shADV-216567
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human NEK1.

shADV-216567
Ad-h-NEK1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NEK1 (NIMA related kinase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ALS24; never in mitosis A-related kinase 1; NIMA (never in mitosis gene a)-related kinase 1; nimA-related protein kinase 1; NY-REN-55; protein-serine/threonine kinase; renal carcinoma antigen NY-REN-55; serine/threonine-protein kinase Nek1; SRPS2; SRPS2A; SRTD6
Description (eCommerce)
Never in mitosis A-related kinase 1 (NEK1) is a serine/threonine kinase involved in cell cycle regulation. NEK1 is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD) [taken from NCBI Entrez Gene (Gene ID: 4750)].
Description (Vector)
NEK1 is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene.
Gene ID
4750
Gene Name (eCommerce)
NIMA related kinase 1
Gene Name (Vector)
NIMA related kinase 1
Gene Symbol
NEK1
HGNC ID
HGNC:7744
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3570
ORF Size (bp)
3570 bp
Protein Name (eCommerce)
Serine/threonine-protein kinase Nek1
RefSeq ID
NM_012224
RefSeq Synonyms
NM_012224, NM_001199400, NM_001199399, NM_001199398, NM_001199397, BC114491,
Research Areas
Cell Cycle,Cell Division,Cytoskeleton,DNA Damage/Repair,Kinase/Phosphatase
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,signal_transduction,transcription_translation
Species
human
Target Sentence
Never in mitosis A-related kinase 1 (NEK1) is a serine/threonine kinase involved in cell cycle regulation. NEK1 is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD) [taken from NCBI Entrez Gene (Gene ID: 4750)].
UniGene ID
Hs.481181
UniProt ID (eCommerce)
Q96PY6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.