human NEFL shRNA silencing Adenovirus

Name 
Ad-h-NEFL-shRNA
Cat No 
shADV-216560
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NEFL.

shADV-216560
Ad-h-NEFL-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NEFL (neurofilament, light polypeptide). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
68 kDa neurofilament protein; CMT1F; CMT2E; CMTDIG; light molecular weight neurofilament protein; neurofilament light polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; NF68; NFL; NF-L; PPP1R110; protein phosphatase 1, regulatory subunit 110
Description (eCommerce)
Neurofilaments are type IV intermediate filament heteropolymers composed of light (L), medium (M), and heavy (H) chains. Neurofilaments are a component of the axon cytoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. Mutations in the Neurofilament-L cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. [taken from NCBI Entrez Gene (Gene ID: 4747)].
Description (Vector)
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Gene ID
4747
Gene Name (eCommerce)
neurofilament light
Gene Name (Vector)
neurofilament, light polypeptide
Gene Symbol
NEFL
HGNC ID
HGNC:7739
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
855
Protein Name (eCommerce)
Neurofilament light polypeptide
RefSeq ID
NM_006158
RefSeq Synonyms
NM_006158, BC066952, BC039237,
Research Areas
Amyotrophic Lateral Sclerosis (ALS),Cytoskeleton,Kinase/Phosphatase,Neurobiology,Neurodegeneration,Neurodevelopment
Research Areas (Faceted)
cell_biology,developmental_biology,neurobiology,signal_transduction
Species
human
Target Sentence
Neurofilaments are type IV intermediate filament heteropolymers composed of light (L), medium (M), and heavy (H) chains. Neurofilaments are a component of the axon cytoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. Mutations in the Neurofilament-L cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. [taken from NCBI Entrez Gene (Gene ID: 4747)].
UniGene ID
Hs.521461
UniProt ID (eCommerce)
P07196

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.