human NDUFAF1 shRNA silencing Adenovirus

Name 
Ad-h-NDUFAF1-shRNA
Cat No 
shADV-216493
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human NDUFAF1.

shADV-216493
Ad-h-NDUFAF1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NDUFAF1 (NADH:ubiquinone oxidoreductase complex assembly factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CGI-65; CGI65; CIA30
Description (Vector)
NDUFAF1 encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19.
Gene ID
51103
Gene Name (Vector)
NADH:ubiquinone oxidoreductase complex assembly factor 1
Gene Symbol
NDUFAF1
HGNC ID
HGNC:18828
ORF Size (aa)
984
ORF Size (bp)
984 bp
RefSeq ID
NM_016013
RefSeq Synonyms
NM_016013, BC000780,
Species
human
UniGene ID
Hs.106529

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.