human NCF4 shRNA silencing Adenovirus

Name
Ad-h-NCF4-shRNA

Cat No
shADV-216399

Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human NCF4.

shADV-216399

Ad-h-NCF4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NCF4 (neutrophil cytosolic factor 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Validated

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

CGD3; NCF; P40PHOX; SH3PXD4

Description (Vector)

NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene ID

4689

Gene Name (Vector)

neutrophil cytosolic factor 4

Gene Symbol

NCF4

HGNC ID

HGNC:7662

ORF Size (aa)

1047

ORF Size (bp)

1047 bp

RefSeq ID

NM_000631

RefSeq Synonyms

NM_013416, NM_000631, BC002798,

Species

human

UniGene ID

Hs.474781

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.