human MPRIP shRNA silencing Adenovirus

Name 
Ad-h-MPRIP-shRNA
Cat No 
shADV-215730
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human MPRIP.

shADV-215730
Ad-h-MPRIP-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human MPRIP (myosin phosphatase Rho interacting protein). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
MRIP; M-RIP; myosin phosphatase Rho-interacting protein; p116Rip; Rho interacting protein 3; Rho-interacting protein 3; RHOIP3; RIP3
Description (eCommerce)
MPRIP targets myosin phosphatase to the actin cytoskeleton. It is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MPRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6WCQ1].
Gene ID
23164
Gene Name (eCommerce)
myosin phosphatase Rho interacting protein
Gene Name (Vector)
myosin phosphatase Rho interacting protein
Gene Symbol
MPRIP
HGNC ID
HGNC:30321
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3114
ORF Size (bp)
3114 bp
Protein Name (eCommerce)
Myosin phosphatase Rho-interacting protein
RefSeq ID
NM_015134
RefSeq Synonyms
NM_201274, NM_015134, BC136479,
Research Areas
Cytoskeleton,EGFR Signaling,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology
Species
human
Target Sentence
MPRIP targets myosin phosphatase to the actin cytoskeleton. It is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MPRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6WCQ1].
UniGene ID
Hs.462341
UniProt ID (eCommerce)
Q6WCQ1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.