human MPI shRNA silencing Adenovirus

Name 
Ad-h-MPI-shRNA
Cat No 
shADV-215711
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human MPI.

shADV-215711
Ad-h-MPI-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human MPI (mannose phosphate isomerase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CDG1B; mannose-6-phosphate isomerase; phosphohexomutase; Phosphomannose isomerase; phosphomannose isomerase 1; PMI; PMI1
Description (eCommerce)
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants [taken from NCBI Entrez Gene (Gene ID: 4351)].
Description (Vector)
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.
Gene ID
4351
Gene Name (eCommerce)
mannose phosphate isomerase
Gene Name (Vector)
mannose phosphate isomerase
Gene Symbol
MPI
HGNC ID
HGNC:7216
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1089
ORF Size (bp)
1089 bp
Protein Name (eCommerce)
Mannose-6-phosphate isomerase
RefSeq ID
NM_002435
RefSeq Synonyms
NM_002435, NM_001330372, NM_001289157, NM_001289156, NM_001289155, BC046357, BC017351,
Research Areas
Metabolism/Metabolic Process,Translational Control
Research Areas (Faceted)
cell_biology,metabolism,transcription_translation
Species
human
Target Sentence
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants [taken from NCBI Entrez Gene (Gene ID: 4351)].
UniGene ID
Hs.75694
UniProt ID (eCommerce)
P34949

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.