human MED21 shRNA silencing Adenovirus

Name 
Ad-h-MED21-shRNA
Cat No 
shADV-215253
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human MED21.

shADV-215253
Ad-h-MED21-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human MED21 (mediator complex subunit 21). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
hSrb7; SRB7; SURB7
Description (Vector)
The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for transcriptional activation of polymerase II (see MIM 180660)-transcribed genes. MED21 is a Mediator subunit predicted to reside in the middle module and appears to be a core subunit found in nearly all Mediator preparations (summary by Sato et al., 2004 (PubMed 15175163)).(supplied by OMIM, Nov 2010)
Gene ID
9412
Gene Name (Vector)
mediator complex subunit 21
Gene Symbol
MED21
HGNC ID
HGNC:11473
ORF Size (aa)
435
RefSeq ID
NM_004264
RefSeq Synonyms
NM_004264, NM_001271811, BC008380,
Species
human
UniGene ID
Hs.286145

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.