human LATS1 shRNA silencing Adenovirus

Name
Ad-h-LATS1-shRNA

Cat No
shADV-213705

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human LATS1.

shADV-213705

Ad-h-LATS1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human LATS1 (large tumor suppressor kinase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

h-warts; large tumor suppressor homolog 1; LATS (large tumor suppressor, Drosophila) homolog 1; LATS, large tumor suppressor, homolog 1; serine/threonine-protein kinase LATS1; WARTS; WARTS protein kinase; wts

Description (eCommerce)

LATS1 (Large Tumor Suppressor homolog 1) has been identified as a tumor suppressor protein linked to sarcomas and ovarian cancer. LATS1 is a member of the Dbf2-related (NDR) family of protein kinases that are key components of the mitotic exit network. LATS1 interacts with MOB1 and CDC2 kinase in early mitosis. LATS1 appears to negatively regulate G2/M transitioning by down-regulating CDC2 kinase activity.

Description (Vector)

LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. Two protein-coding transcripts and one non-protein coding transcript have been found for this gene.

Gene ID

9113

Gene Name (eCommerce)

large tumor suppressor kinase 1

Gene Name (Vector)

large tumor suppressor kinase 1

Gene Symbol

LATS1

HGNC ID

HGNC:6514

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2073

ORF Size (bp)

2073 bp

Protein Name (eCommerce)

Serine/threonine-protein kinase LATS1

RefSeq ID

NM_004690

RefSeq Synonyms

NM_004690, NM_001270519, BC015665, BC002767,

Research Areas

Cancer,Cell Cycle,Cell Division,Cytoskeleton,Hippo Signaling,Kinase/Phosphatase,Signal Transduction,Tumor Suppressors/Oncoproteins,Wnt Signaling

Research Areas (Faceted)

cancer,cell_biology,cell_cycle,genetics,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.716697

UniProt ID (eCommerce)

O95835

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.