human KIAA0196 shRNA silencing Adenovirus

Name 
Ad-h-KIAA0196-shRNA
Cat No 
shADV-212893
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human WASHC5.

shADV-212893
Ad-h-KIAA0196-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human WASHC5 (WASH complex subunit 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
KIAA0196; RTSC; RTSC1; SPG8; strumpellin; WASH complex subunit 5; WASH complex subunit strumpellin
Description (eCommerce)
SPG8/Strumpellin acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093). SPG8/Strumpellin may be involved in axonal outgrowth [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q12768].
Description (Vector)
KIAA0196 encodes a 134 kDa protein named strumpellin that is predicted to have multiple transmembrane domains and a spectrin-repeat-containing domain. This ubiquitously expressed gene has its highest expression in skeletal muscle. The protein is named for Strumpell disease; a form of hereditary spastic paraplegia (HSP). Spastic paraplegias are a diverse group of disorders in which the autosomal dominant forms are characterized by progressive, lower extremity spasticity caused by axonal degeneration in the terminal portions of the longest descending and ascending corticospinal tracts. More than 30 loci (SPG1-33) have been implicated in hereditary spastic paraplegia diseases.
Gene ID
9897
Gene Name (eCommerce)
WASH complex subunit 5
Gene Name (Vector)
WASH complex subunit 5
Gene Symbol
WASHC5
HGNC ID
HGNC:28984
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3480
ORF Size (bp)
3480 bp
Protein Name (eCommerce)
WASH complex subunit strumpellin
RefSeq ID
NM_014846
RefSeq Synonyms
NM_014846, NM_001330609, BC106015, BC026951,
Research Areas
Apoptosis,Cell Biology,Neurobiology,Neurodegeneration
Research Areas (Faceted)
cell_biology,neurobiology
Species
human
Target Sentence
SPG8/Strumpellin acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093). SPG8/Strumpellin may be involved in axonal outgrowth [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q12768].
UniGene ID
Hs.270043
UniProt ID (eCommerce)
Q12768

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.