human ILK shRNA silencing Adenovirus

Name 
Ad-h-ILK-shRNA
Cat No 
shADV-212226
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ILK.

shADV-212226
Ad-h-ILK-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ILK (integrin linked kinase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
59 kDa serine/threonine-protein kinase; epididymis secretory protein Li 28; HEL-S-28; ILK-1; ILK-2; integrin-linked kinase-2; integrin-linked protein kinase; P59; p59ILK
Description (eCommerce)
Integrin-linked kinase (ILK) is a serine/threonine kinase associated with the cytoplasmic domain of beta integrins and mediates integrin signaling from the extracellular matrix to the intracellular actin cytoskeleton. PKB/Akt and GSK-3beta are targets of ILK involved in integrin-mediated cell proliferation and cell survival. ILK has been shown to interact with Paxillin, PINCH-1, PINCH-2, and parvin to influence actin dynamics.
Description (Vector)
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Gene ID
3611
Gene Name (eCommerce)
integrin linked kinase
Gene Name (Vector)
integrin linked kinase
Gene Symbol
ILK
HGNC ID
HGNC:6040
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1359
ORF Size (bp)
1359 bp
Protein Name (eCommerce)
Integrin-linked protein kinase
RefSeq ID
NM_004517
RefSeq Synonyms
NM_004517, NM_001278442, NM_001278441, NM_001014795, NM_001014794, BC001554,
Research Areas
Aging,Angiogenesis,Apoptosis,Cancer,Cardiac Development,Cardiology,Cardiovascular,Cell Cycle,Cytoskeleton,Kinase/Phosphatase,MAPK Signaling,Neurobiology,Neurodevelopment,Signal Transduction,Wnt Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,neurobiology,signal_transduction
Species
human
Target Sentence
Integrin-linked kinase (ILK) is a serine/threonine kinase associated with the cytoplasmic domain of beta integrins and mediates integrin signaling from the extracellular matrix to the intracellular actin cytoskeleton. PKB/Akt and GSK-3beta are targets of ILK involved in integrin-mediated cell proliferation and cell survival. ILK has been shown to interact with Paxillin, PINCH-1, PINCH-2, and parvin to influence actin dynamics.
UniGene ID
Hs.706355
UniProt ID (eCommerce)
Q13418

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.