human HIRA shRNA silencing Adenovirus

Name 
Ad-h-HIRA-shRNA
Cat No 
shADV-211046
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human HIRA.

shADV-211046
Ad-h-HIRA-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human HIRA (histone cell cycle regulator). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DGCR1; TUP1; TUPLE1
Description (Vector)
HIRA encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. HIRA plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
Gene ID
7290
Gene Name (Vector)
histone cell cycle regulator
Gene Symbol
HIRA
HGNC ID
HGNC:4916
ORF Size (aa)
3054
ORF Size (bp)
3054 bp
RefSeq ID
NM_003325
RefSeq Synonyms
NM_003325, BC039835,
Species
human
UniGene ID
Hs.474206

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.