human GPT shRNA silencing Adenovirus

Name
Ad-h-GPT-shRNA

Cat No
shADV-210451

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human GPT.

shADV-210451

Ad-h-GPT-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human GPT (glutamic–pyruvic transaminase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

AAT1; ALT1; GPT1

Description (Vector)

GPT encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

Gene ID

2875

Gene Name (Vector)

glutamic--pyruvic transaminase

Gene Symbol

GPT

HGNC ID

HGNC:4552

ORF Size (aa)

1491

ORF Size (bp)

1491 bp

RefSeq ID

NM_005309

RefSeq Synonyms

NM_005309, BC018207,

Species

human

UniGene ID

Hs.103502

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.