human GPI shRNA silencing Adenovirus

Name 
Ad-h-GPI-shRNA
Cat No 
shADV-210233
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human GPI.

shADV-210233
Ad-h-GPI-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human GPI (glucose-6-phosphate isomerase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AMF; autocrine motility factor; glucose-6-phosphate isomerase; GNPI; GPI; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; NLK; oxoisomerase; PGI; PHI; phosphoglucose isomerase; phosphohexomutase; phosphohexose isomerase; phosphosaccharomutase; SA36; SA-36; Sperm antigen 36; sperm antigen-36
Description (eCommerce)
Glucose-6-phosphate isomerase (GPI) is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. GPI functions in different capacities inside and outside the cell. In the cytoplasm, GPI is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in the GPI gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment [taken from NCBI Entrez Gene (Gene ID: 2821)].
Description (Vector)
GPI belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Gene ID
2821
Gene Name (eCommerce)
glucose-6-phosphate isomerase
Gene Name (Vector)
glucose-6-phosphate isomerase
Gene Symbol
GPI
HGNC ID
HGNC:4458
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1677
ORF Size (bp)
1677 bp
Protein Name (eCommerce)
Glucose-6-phosphate isomerase
RefSeq ID
NM_000175
RefSeq Synonyms
NM_001329911, NM_001329910, NM_001329909, NM_001289790, NM_001289789, NM_001184722, NM_000175, BC004982,
Research Areas
Angiogenesis,Apoptosis,Immunology,Metabolism/Metabolic Process,Neurobiology,Neurodevelopment
Research Areas (Faceted)
cancer,cell_biology,developmental_biology,immunology,metabolism,neurobiology
Species
human
Target Sentence
Glucose-6-phosphate isomerase (GPI) is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. GPI functions in different capacities inside and outside the cell. In the cytoplasm, GPI is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in the GPI gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment [taken from NCBI Entrez Gene (Gene ID: 2821)].
UniGene ID
Hs.466471
UniProt ID (eCommerce)
P06744

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.