human FUSIP1 shRNA silencing Adenovirus

Name 
Ad-h-FUSIP1-shRNA
Cat No 
shADV-209413
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SRSF10.

shADV-209413
Ad-h-FUSIP1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SRSF10 (serine and arginine rich splicing factor 10). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
50
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
40 kDa SR-repressor protein; FUS-interacting protein (serine-arginine rich) 2; FUS-interacting serine-arginine-rich protein 1; FUSIP1; FUSIP2; neural-salient SR protein; NSSR; PPP1R149; protein phosphatase 1, regulatory subunit 149; serine/arginine-rich splicing factor 10; serine-arginine repressor protein (40 kDa); SFRS13; SFRS13A; splicing factor SRp38; splicing factor, arginine/serine-rich 13; splicing factor, arginine/serine-rich 13A; SR splicing factor 10; SRp38; SRrp40; TASR; TASR1; TASR2; TLS-associated protein TASR; TLS-associated protein with Ser-Arg repeats; TLS-associated protein with SR repeats; TLS-associated serine-arginine protein
Description (eCommerce)
FUSIP1 (FUS-interacting protein 1) was originally identified in a two-hybrid screen for partners of the TLS (translocated in liposarcoma) protein also known as FUS. FUSIP1 is a splicing factor of the serine-arginine (SR) family of proteins. It functions as a splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. FUSIP1 seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70, and it is required for splicing repression in M-phase cells and after heat shock [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75494].
Description (Vector)
SRSF10 product is a member of the serine-arginine (SR) family of proteins, which is involved in constitutive and regulated RNA splicing. Members of this family are characterized by N-terminal RNP1 and RNP2 motifs, which are required for binding to RNA, and multiple C-terminal SR/RS repeats, which are important in mediating association with other cellular proteins. This protein can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS, and abrogates the influence of TLS on E1A pre-mRNA splicing. SRSF10 has multiple pseudogenes. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. In addition, transcript variants utilizing alternative polyA sites exist.
Gene ID
10772
Gene Name (eCommerce)
serine and arginine rich splicing factor 10
Gene Name (Vector)
serine and arginine rich splicing factor 10
Gene Symbol
SRSF10
HGNC ID
HGNC:16713
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
522
Protein Name (eCommerce)
Serine/arginine-rich splicing factor 10
RefSeq ID
NM_006625
RefSeq Synonyms
NM_054016, NM_006625, NM_001300937, NM_001300936, NM_001191009, NM_001191007, NM_001191006, NM_001191005, BC010074, BC005039, BC001107,
Research Areas
Cancer,Gene Expression,Immunology,Kinase/Phosphatase,mRNA Processing,mRNA Splicing,Protein Folding,RNA Binding,RNA Processing,Spliceosome,Transcription Factor/Regulator,Tumor Suppressors/Oncoproteins
Research Areas (Faceted)
cancer,cell_biology,genetics,immunology,signal_transduction,transcription_translation
Species
human
Target Sentence
FUSIP1 (FUS-interacting protein 1) was originally identified in a two-hybrid screen for partners of the TLS (translocated in liposarcoma) protein also known as FUS. FUSIP1 is a splicing factor of the serine-arginine (SR) family of proteins. It functions as a splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. FUSIP1 seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70, and it is required for splicing repression in M-phase cells and after heat shock [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75494].
UniGene ID
Hs.652334
UniProt ID (eCommerce)
O75494

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.