human ETF1 shRNA silencing Adenovirus

Name 
Ad-h-ETF1-shRNA
Cat No 
shADV-208132
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human ETF1.

shADV-208132
Ad-h-ETF1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ETF1 (eukaryotic translation termination factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
D5S1995; ERF; ERF1; RF1; SUP45L1; TB3-1
Description (Vector)
ETF1 encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X.
Gene ID
2107
Gene Name (Vector)
eukaryotic translation termination factor 1
Gene Symbol
ETF1
HGNC ID
HGNC:3477
ORF Size (aa)
1314
ORF Size (bp)
1314 bp
RefSeq ID
NM_004730
RefSeq Synonyms
NM_004730, NM_001291975, NM_001291974, NM_001282185, NM_001256302, BC088358, BC014269,
Species
human
UniGene ID
Hs.483494

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.