human DHCR24 shRNA silencing Adenovirus

Name
Ad-h-DHCR24-shRNA

Cat No
shADV-206930

Availability
Immediate

This is an Adenovirus expressing shRNA for silencing of Human DHCR24.

shADV-206930

Ad-h-DHCR24-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human DHCR24 (24-dehydrocholesterol reductase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

DCE; Nbla03646; seladin-1; SELADIN1

Description (Vector)

DHCR24 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.

Gene ID

1718

Gene Name (Vector)

24-dehydrocholesterol reductase

Gene Symbol

DHCR24

HGNC ID

HGNC:2859

ORF Size (aa)

1551

ORF Size (bp)

1551 bp

RefSeq ID

NM_014762

RefSeq Synonyms

NM_014762, BC011669, BC004375,

Species

human

UniGene ID

Hs.498727

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.