human DGKA shRNA silencing Adenovirus

Name 
Ad-h-DGKA-shRNA
Cat No 
shADV-206919
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human DGKA.

shADV-206919
Ad-h-DGKA-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human DGKA (diacylglycerol kinase alpha). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DAGK; DAGK1; DGK-alpha
Description (Vector)
DGKA belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Gene ID
1606
Gene Name (Vector)
diacylglycerol kinase alpha
Gene Symbol
DGKA
HGNC ID
HGNC:2849
ORF Size (aa)
2208
ORF Size (bp)
2208 bp
RefSeq ID
NM_001345
RefSeq Synonyms
NM_201554, NM_201445, NM_201444, NM_001345, BC031870, BC023523,
Species
human
UniGene ID
Hs.524488

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.