human DFFA shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human DFFA.
shADV-206894
Ad-h-DFFA-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human DFFA (DNA fragmentation factor subunit alpha). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
DFF1; DFF45; DFF-45; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; inhibitor of CAD
Description (eCommerce)
DFF (DNA fragmentation factor) is the principle endonuclease responsible for DNA cleavage during apoptosis and exists as a heterodimer formed between the inhibitory subunit, DFF45, and the DNA nuclease, DFF40. DFF35/DFFA is a 35 kDa splice variant of the inhibitory subunit, DFF45. Similar to DFF45, DFF35 has been shown to heterodimerize with DFF40 and also negatively regulate its nuclease activity. In addition to regulating DFF40 activity, DFF35 has also been found to assist in the synthesis and folding of the DFF40 endonuclease.
Description (Vector)
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Gene ID
1676
Gene Name (eCommerce)
DNA fragmentation factor subunit alpha
Gene Name (Vector)
DNA fragmentation factor subunit alpha
Gene Symbol
DFFA
HGNC ID
HGNC:2772
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
996
ORF Size (bp)
996 bp
Protein Name (eCommerce)
DNA fragmentation factor subunit alpha
RefSeq ID
NM_004401
RefSeq Synonyms
NM_213566, NM_004401, BC007721, BC007112, BC000037,
Research Areas
Apoptosis,Cancer,CD95/FAS Signaling,Host-Virus Interactions,Signal Transduction
Research Areas (Faceted)
cancer,signal_transduction,cell_biology,infectious_disease
Species
human
Target Sentence
DFF (DNA fragmentation factor) is the principle endonuclease responsible for DNA cleavage during apoptosis and exists as a heterodimer formed between the inhibitory subunit, DFF45, and the DNA nuclease, DFF40. DFF35/DFFA is a 35 kDa splice variant of the inhibitory subunit, DFF45. Similar to DFF45, DFF35 has been shown to heterodimerize with DFF40 and also negatively regulate its nuclease activity. In addition to regulating DFF40 activity, DFF35 has also been found to assist in the synthesis and folding of the DFF40 endonuclease.
UniGene ID
Hs.484782
UniProt ID (eCommerce)
O00273
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.