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human CPSF4 shRNA silencing Adenovirus

Name 
Ad-h-CPSF4-shRNA
Cat No 
shADV-205932
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human CPSF4.

shADV-205932
Ad-h-CPSF4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CPSF4 (cleavage and polyadenylation specific factor 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
cleavage and polyadenylation specific factor 4, 30kDa; cleavage and polyadenylation specificity factor 30 kDa subunit; cleavage and polyadenylation specificity factor subunit 4; CPSF 30 kDa subunit; CPSF30; NAR; NEB1; NEB-1; no arches homolog; no arches-like zinc finger protein; NS1 effector domain-binding protein 1
Description (eCommerce)
CPSF30/CPSF4 (cleavage and polyadenylation specific factor 4, 30 kDa subunit) is a subunit of the cleavage and polyadenylation specificity factor (CPSF) involved in mammalian mRNA 3’-end processing. CPSF, along with CstF (cleavage and stimulation factor), is critical for the recognition of cis-acting sequence elements on the pre-mRNA that dictate the site of cleavage and subsequent polyadenylation. CPSF exists as a complex of four proteins that includes CPSF160/CPSF1, CPSF100/CPSF2, CPSF73/CPSF3, and CPSF30/CPSF4. Studies of the Arabidopsis CPSF30/CPSF4 ortholog have revealed that CPSF30/CPSF4 may function as a processing endonuclease and interacts with Fip1, an integral subunit of CPSF.
Description (Vector)
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.
Gene ID
10898
Gene Name (eCommerce)
cleavage and polyadenylation specific factor 4
Gene Name (Vector)
cleavage and polyadenylation specific factor 4
Gene Symbol
CPSF4
HGNC ID
HGNC:2327
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
732
ORF Size (bp)
732 bp
Protein Name (eCommerce)
Cleavage and polyadenylation specificity factor subunit 4
RefSeq ID
NM_006693
RefSeq Synonyms
NM_006693, NM_001318162, NM_001318161, NM_001318160, NM_001081559, BC050738, BC003101,
Research Areas
Host-Virus Interactions,Mitochondrion,mRNA Processing,RNA Binding,RNA Processing,Zinc-finger
Research Areas (Faceted)
cell_biology,genetics,infectious_disease,transcription_translation
Species
human
Target Sentence
CPSF30/CPSF4 (cleavage and polyadenylation specific factor 4, 30 kDa subunit) is a subunit of the cleavage and polyadenylation specificity factor (CPSF) involved in mammalian mRNA 3’-end processing. CPSF, along with CstF (cleavage and stimulation factor), is critical for the recognition of cis-acting sequence elements on the pre-mRNA that dictate the site of cleavage and subsequent polyadenylation. CPSF exists as a complex of four proteins that includes CPSF160/CPSF1, CPSF100/CPSF2, CPSF73/CPSF3, and CPSF30/CPSF4. Studies of the Arabidopsis CPSF30/CPSF4 ortholog have revealed that CPSF30/CPSF4 may function as a processing endonuclease and interacts with Fip1, an integral subunit of CPSF.
UniGene ID
Hs.489287
UniProt ID (eCommerce)
O95639

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.