human CISH shRNA silencing Adenovirus

Name 
Ad-h-CISH-shRNA
Cat No 
shADV-205295
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human CISH.

shADV-205295
Ad-h-CISH-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CISH (cytokine inducible SH2 containing protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
BACTS2; CIS; CIS-1; G18; SOCS
Description (Vector)
CISH contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
1154
Gene Name (Vector)
cytokine inducible SH2 containing protein
Gene Symbol
CISH
HGNC ID
HGNC:1984
ORF Size (aa)
777
ORF Size (bp)
777 bp
RefSeq ID
NM_013324
RefSeq Synonyms
NM_145071, NM_013324, BC064354, BC031590,
Species
human
UniGene ID
Hs.655334

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.