human CHRNA4 shRNA silencing Adenovirus

Name
Ad-h-CHRNA4-shRNA

Cat No
shADV-205213

Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human CHRNA4.

shADV-205213

Ad-h-CHRNA4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CHRNA4 (cholinergic receptor nicotinic alpha 4 subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Validated

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

BFNC; EBN; EBN1; NACHR; NACHRA4; NACRA4

Description (Vector)

CHRNA4 encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants.

Gene ID

1137

Gene Name (Vector)

cholinergic receptor nicotinic alpha 4 subunit

Gene Symbol

CHRNA4

HGNC ID

HGNC:1958

ORF Size (aa)

1671

ORF Size (bp)

1671 bp

RefSeq ID

NM_000744

RefSeq Synonyms

NM_001256573, NM_000744, BC096293, BC096292, BC096291, BC096290,

Species

human

UniGene ID

Hs.10734

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.