human CHCHD4 shRNA silencing Adenovirus

Name 
Ad-h-CHCHD4-shRNA
Cat No 
shADV-205104
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human CHCHD4.

shADV-205104
Ad-h-CHCHD4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CHCHD4 (coiled-coil-helix-coiled-coil-helix domain containing 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
coiled-coil-helix-coiled-coil-helix domain-containing protein 4; MIA40; mitochondrial intermembrane space import and assembly protein 40; TIMM40; translocase of inner mitochondrial membrane 40 homolog
Description (eCommerce)
CHCHD4 functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19 and MICU1. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system. Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8N4Q1].
Description (Vector)
CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 (PubMed 16185709)).(supplied by OMIM, Mar 2008)
Gene ID
131474
Gene Name (eCommerce)
coiled-coil-helix-coiled-coil-helix domain containing 4
Gene Name (Vector)
coiled-coil-helix-coiled-coil-helix domain containing 4
Gene Symbol
CHCHD4
HGNC ID
HGNC:26467
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
429
ORF Size (bp)
429 bp
Protein Name (eCommerce)
Mitochondrial intermembrane space import and assembly protein 40
RefSeq ID
NM_144636
RefSeq Synonyms
NM_144636, NM_001098502, BC033775, BC017082,
Research Areas
Metabolism/Metabolic Process,Mitochondrion
Research Areas (Faceted)
cell_biology,metabolism
Species
human
Target Sentence
CHCHD4 functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19 and MICU1. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system. Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8N4Q1].
UniGene ID
Hs.517815
UniProt ID (eCommerce)
Q8N4Q1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.