human CDK4 shRNA silencing Adenovirus

Name
Ad-h-CDK4-shRNA

Cat No
shADV-204844

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human CDK4.

shADV-204844

Ad-h-CDK4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CDK4 (cyclin dependent kinase 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

cell division protein kinase 4; CMM3; cyclin-dependent kinase 4; PSK-J3

Description (eCommerce)

Cyclin dependent kinase 4 (CDK4) is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of CDK4 is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in the CDK4 gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers [taken from NCBI Entrez Gene (Gene ID: 1019)].

Description (Vector)

CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Gene ID

1019

Gene Name (eCommerce)

cyclin dependent kinase 4

Gene Name (Vector)

cyclin dependent kinase 4

Gene Symbol

CDK4

HGNC ID

HGNC:1773

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

912

ORF Size (bp)

912 bp

Protein Name (eCommerce)

Cyclin-dependent kinase 4

RefSeq ID

NM_000075

RefSeq Synonyms

NM_000075, BC010153, BC005864, BC003644,

Research Areas

B-cell Receptor Signaling,Bladder Cancer,Breast Cancer,Cancer,Cell Cycle,Cell Division,DNA Damage/Repair,EPO Signaling,Gene Expression,Glioma,Host-Virus Interactions,Immunology,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,Leukemia,Lung Cancer,Neurobiology,p53 Signaling,PI3K/Akt Signaling,Prostate Cancer,Signal Transduction,T-cell Receptor Signaling,TGF-beta Signaling,Transcription Factor/Regulator,TSH Signaling,VEGF Signaling,Viral Carcinogenesis

Research Areas (Faceted)

cancer,cell_biology,cell_cycle,genetics,transcription_translation,immunology,infectious_disease,neurobiology,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.95577

UniProt ID (eCommerce)

P11802

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.