human C22ORF28 shRNA silencing Adenovirus

Name 
Ad-h-C22ORF28-shRNA
Cat No 
shADV-203266
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RTCB.

shADV-203266
Ad-h-C22ORF28-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RTCB (RNA 2′,3′-cyclic phosphate and 5′-OH ligase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ankyrin repeat domain 54; C22orf28; DJ149A16.6; FAAP; focal adhesion-associated protein; HSPC117; tRNA-splicing ligase RtcB homolog
Description (eCommerce)
RTCB encodes tRNA-splicing ligase RtcB homolog, the catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs. tRNA-splicing ligase RtcB homolog may also act as an RNA ligase with broad substrate specificity, and may function toward other RNAs [taken from UniProtKB (Entry: Q9Y3I0)].
Gene ID
51493
Gene Name (eCommerce)
RNA 2',3'-cyclic phosphate and 5'-OH ligase
Gene Name (Vector)
RNA 2',3'-cyclic phosphate and 5'-OH ligase
Gene Symbol
RTCB
HGNC ID
HGNC:26935
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1518
ORF Size (bp)
1518 bp
Protein Name (eCommerce)
tRNA-splicing ligase RtcB homolog
RefSeq ID
NM_014306
RefSeq Synonyms
NM_014306, BC016707, BC010308, BC002970, BC000151,
Research Areas
RNA Processing
Research Areas (Faceted)
cell_biology,genetics,transcription_translation
Species
human
Target Sentence
RTCB encodes tRNA-splicing ligase RtcB homolog, the catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs. tRNA-splicing ligase RtcB homolog may also act as an RNA ligase with broad substrate specificity, and may function toward other RNAs [taken from UniProtKB (Entry: Q9Y3I0)].
UniGene ID
Hs.474643
UniProt ID (eCommerce)
Q9Y3I0

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.