human C11ORF59 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human LAMTOR1.
shADV-202489
Ad-h-C11ORF59-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human LAMTOR1 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
C11orf59; late endosomal/lysosomal adaptor and MAPK and MTOR activator 1; lipid raft adaptor protein p18; p18; p27kip1 releasing factor from RhoA; p27Kip1-releasing factor from RhoA; p27RF-Rho; PDRO; protein associated with DRMs and endosomes; ragulator complex protein LAMTOR1; ragulator complex protein PDRO; Ragulator1; RhoA activator C11orf59
Description (eCommerce)
As part of the Ragulator complex, LAMTOR1 is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. LAMTOR1 is activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes. It is also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. LAMTOR1 may be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. It may also play a role in RHOA activation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6IAA8].
Gene ID
55004
Gene Name (eCommerce)
late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
Gene Name (Vector)
late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
Gene Symbol
LAMTOR1
HGNC ID
HGNC:26068
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
486
ORF Size (bp)
486 bp
Protein Name (eCommerce)
Ragulator complex protein LAMTOR1
RefSeq ID
NM_017907
RefSeq Synonyms
NM_017907, BC001706,
Research Areas
Golgi Apparatus,Lysosome,MAPK Signaling,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology
Species
human
Target Sentence
As part of the Ragulator complex, LAMTOR1 is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. LAMTOR1 is activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes. It is also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. LAMTOR1 may be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. It may also play a role in RHOA activation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6IAA8].
UniGene ID
Hs.530753
UniProt ID (eCommerce)
Q6IAA8
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.