human ARFGEF1 shRNA silencing Adenovirus

Name 
Ad-h-ARFGEF1-shRNA
Cat No 
shADV-201284
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ARFGEF1.

shADV-201284
Ad-h-ARFGEF1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ARFGEF1 (ADP ribosylation factor guanine nucleotide exchange factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ADP-ribosylation factor guanine nucleotide-exchange factor 1; ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited); ARFGEP1; BIG1; Brefeldin A-inhibited GEP 1; brefeldin A-inhibited guanine nucleotide-exchange protein 1; P200; p200 ARF guanine nucleotide exchange factor; p200 ARF-GEP1
Description (eCommerce)
BIG1/ARFGEF1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.
Description (Vector)
ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for guanine-nucleotide exchange activity and also brefeldin A inhibition.
Gene ID
10565
Gene Name (eCommerce)
ADP ribosylation factor guanine nucleotide exchange factor 1
Gene Name (Vector)
ADP ribosylation factor guanine nucleotide exchange factor 1
Gene Symbol
ARFGEF1
HGNC ID
HGNC:15772
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
5547
ORF Size (bp)
5547 bp
Protein Name (eCommerce)
Brefeldin A-inhibited guanine nucleotide-exchange protein 1
RefSeq ID
NM_006421
RefSeq Synonyms
NM_006421, BC172243,
Research Areas
Golgi Apparatus,Signal Transduction
Research Areas (Faceted)
cell_biology,signal_transduction
Species
human
Target Sentence
BIG1/ARFGEF1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.
UniGene ID
Hs.656902
UniProt ID (eCommerce)
Q9Y6D6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.