human AKAP13 shRNA silencing Adenovirus

Name 
Ad-h-AKAP13-shRNA
Cat No 
shADV-200655
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human AKAP13.

shADV-200655
Ad-h-AKAP13-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human AKAP13 (A-kinase anchoring protein 13). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
A kinase (PRKA) anchor protein 13; AKAP-13; AKAP-Lbc; A-kinase anchor protein 13; ARHGEF13; breast cancer nuclear receptor-binding auxiliary protein; BRX; c-lbc; guanine nucleotide exchange factor Lbc; HA-3; Ht31; human thyroid-anchoring protein 31; LBC; LBC oncogene; lymphoid blast crisis oncogene; non-oncogenic Rho GTPase-specific GTP exchange factor; p47; PRKA13; protein kinase A-anchoring protein 13; PROTO-LB; PROTO-LBC
Description (eCommerce)
AKAP13/AKAP-Lbc was originally identified as an oncogenic protein that associates with the GTP-binding protein Rho. AKAP13-Lbc is also a member of the A-kinase anchoring protein family that functions as a molecular scaffold for the subcellular compartmentalization of A- kinase signaling molecules. AKAP13-Lbc has been found to possess Rho-specific guanine nucleotide exchange factor (GEF) activity and is critical to mediating RhoA activation and signal transduction.
Description (Vector)
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway, function as protein kinase A-anchoring proteins and, in addition, enhance ligand-dependent activity of estrogen receptors alpha and beta.
Gene ID
11214
Gene Name (eCommerce)
A-kinase anchoring protein 13
Gene Name (Vector)
A-kinase anchoring protein 13
Gene Symbol
AKAP13
HGNC ID
HGNC:371
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
8454
ORF Size (bp)
8454 bp
Protein Name (eCommerce)
A-kinase anchor protein 13
RefSeq ID
NM_006738
RefSeq Synonyms
NM_144767, NM_007200, NM_006738, NM_001270546, BC172356, BC050312,
Research Areas
Apoptosis,Cancer,GPCR Signaling,Neurobiology,NGF Signaling,Rho Signaling,Signal Transduction,Zinc-finger
Research Areas (Faceted)
cancer,cell_biology,neurobiology,signal_transduction
Species
human
Target Sentence
AKAP13/AKAP-Lbc was originally identified as an oncogenic protein that associates with the GTP-binding protein Rho. AKAP13-Lbc is also a member of the A-kinase anchoring protein family that functions as a molecular scaffold for the subcellular compartmentalization of A- kinase signaling molecules. AKAP13-Lbc has been found to possess Rho-specific guanine nucleotide exchange factor (GEF) activity and is critical to mediating RhoA activation and signal transduction.
UniGene ID
Hs.710656
UniProt ID (eCommerce)
Q12802

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.