human ACTN4 shRNA silencing Adenovirus

Name
Ad-h-ACTN4-shRNA

Cat No
shADV-200305

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human ACTN4.

shADV-200305

Ad-h-ACTN4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ACTN4 (actinin alpha 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

ACTININ-4; alpha-actinin-4; focal segmental glomerulosclerosis 1; FSGS; FSGS1; non-muscle alpha-actinin 4

Description (eCommerce)

Alpha Actinin 4 / ACTN4 is an F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). It is probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). It is involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. It links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). alpha Actinin 4 / ACTN4 may also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43707].

Description (Vector)

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Gene ID

Gene Name (eCommerce)

actinin alpha 4

Gene Name (Vector)

actinin alpha 4

Gene Symbol

ACTN4

HGNC ID

HGNC:166

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2736

ORF Size (bp)

2736 bp

Protein Name (eCommerce)

Alpha-actinin-4

RefSeq ID

NM_004924

RefSeq Synonyms

NM_004924, NM_001322033, BC005033,

Research Areas

Apoptosis,Cancer,Cardiology,Cardiomyopathy,Cardiovascular,Cytoskeleton,EGFR Signaling,Host-Virus Interactions,Hypoxia,Immunology,PDGF Signaling,Signal Transduction,Viral Carcinogenesis

Research Areas (Faceted)

cancer,cardiovascular,cell_biology,immunology,infectious_disease,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.270291

UniProt ID (eCommerce)

O43707

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.