human ACTL6A shRNA silencing Adenovirus

Name
Ad-h-ACTL6A-shRNA

Cat No
shADV-200294

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human ACTL6A.

shADV-200294

Ad-h-ACTL6A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ACTL6A (actin like 6A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

53 kDa BRG1-associated factor A; actin-like protein 6A; actin-related protein 4; actin-related protein Baf53a; ACTL6; Arp4; arpNbeta; ARPN-BETA; BAF complex 53 kDa subunit; BAF53; BAF53A; BRG1-associated factor 53A; hArpN beta; INO80 complex subunit K; INO80K

Description (eCommerce)

BAF53A is a 53kd subunit of the BAF (BRG1/brm-associated factor) complex, a SWI/SNF chromatin remodeling and histone acetyltransferase complex that functions in the regulation of gene transcription. In the BAF complex, BAF53A is important to the ATPase activity of SMARCA4/BRG1 and the association of the BAF complex with chromatin. BAF53A also appears to be important to the formation of higher-order chromatin structure and has been shown to associate with mitotic chromosomes. Recently, BAF53A has been found to interact with p53 and repress p53-mediated gene transcription in a BRG1-independent manner.

Description (Vector)

ACTL6A encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. ACTL6A encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Gene ID

Gene Name (eCommerce)

actin like 6A

Gene Name (Vector)

actin like 6A

Gene Symbol

ACTL6A

HGNC ID

HGNC:24124

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1164

ORF Size (bp)

1164 bp

Protein Name (eCommerce)

Actin-like protein 6A

RefSeq ID

NM_177989

RefSeq Synonyms

NM_178042, NM_177989, NM_004301, BC036371, BC001391, BC000949,

Research Areas

Cancer,Chromatin Remodeling,DNA Damage/Repair,Histone Modification,Neurobiology,Neurodevelopment,Neurogenesis,Prostate Cancer,Signal Transduction,TNF Signaling,Transcription Factor/Regulator

Research Areas (Faceted)

cancer,signal_transduction,cell_biology,genetics,transcription_translation,neurobiology,developmental_biology,signal_transduction,genetics,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.435326

UniProt ID (eCommerce)

O96019

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.