rat SCAP shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Rat Scap.
shAAV-295178
AAV-r-SCAP-shRNA
Ready-to-use AAV expressing shRNA for silencing of Rat Scap (SREBF chaperone). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).
Gene ID
301024
Gene Name (Vector)
SREBF chaperone
Gene Symbol
Scap
ORF Size (aa)
3831
ORF Size (bp)
3831 bp
RefSeq ID
NM_001100966
RefSeq Synonyms
NM_001100966, BC131852,
Species
rat
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.