rat CCDC99 shRNA silencing AAV

Name
AAV-r-CCDC99-shRNA

Cat No
shAAV-294880

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Rat Spdl1.

shAAV-294880

AAV-r-CCDC99-shRNA

Ready-to-use AAV expressing shRNA for silencing of Rat Spdl1 (spindle apparatus coiled-coil protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Ccdc99; RGD1306908

Description (Vector)

Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.

Gene ID

303037

Gene Name (Vector)

spindle apparatus coiled-coil protein 1

Gene Symbol

Spdl1

ORF Size (aa)

1794

ORF Size (bp)

1794 bp

RefSeq ID

NM_001034138

RefSeq Synonyms

NM_001034138, BC105812,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.