rat DKC1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Rat Dkc1.
shAAV-294458
AAV-r-DKC1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Rat Dkc1 (dyskerin pseudouridine synthase 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Nap57
Description (Vector)
Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme|Function: Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).
Gene ID
170944
Gene Name (Vector)
dyskerin pseudouridine synthase 1
Gene Symbol
Dkc1
ORF Size (aa)
1530
ORF Size (bp)
1530 bp
RefSeq ID
NM_133419
RefSeq Synonyms
NM_133419, BC099832,
Species
rat
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.