mouse DKC1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Dkc1.
shAAV-257205
AAV-m-DKC1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Dkc1 (dyskeratosis congenita 1, dyskerin). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
BC068171
Description (Vector)
Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme|Function: Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).
Gene ID
245474
Gene Name (Vector)
dyskeratosis congenita 1, dyskerin
Gene Symbol
Dkc1
ORF Size (aa)
1530
ORF Size (bp)
1530 bp
RefSeq ID
NM_001030307
RefSeq Synonyms
NM_001030307, BC099966,
Species
mouse
UniGene ID
Mm.291062
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.