rat ATP5J shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Rat Atp5j.
shAAV-290059
AAV-r-ATP5J-shRNA
Ready-to-use AAV expressing shRNA for silencing of Rat Atp5j (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
Gene ID
94271
Gene Name (Vector)
ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
Gene Symbol
Atp5j
ORF Size (aa)
327
RefSeq ID
NM_053602
RefSeq Synonyms
NM_053602, BC059121,
Species
rat
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.