mouse AKAP10 shRNA silencing AAV

Name 
AAV-m-AKAP10-shRNA
Cat No 
shAAV-280538
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Akap10.

shAAV-280538
AAV-m-AKAP10-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Akap10 (A kinase (PRKA) anchor protein 10). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1500031L16Rik; B130049N18Rik; D-AKAP-2; D-AKAP2; PRKA10
Description (Vector)
Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase (By similarity).
Gene ID
56697
Gene Name (Vector)
A kinase (PRKA) anchor protein 10
Gene Symbol
Akap10
ORF Size (aa)
1119
ORF Size (bp)
1119 bp
RefSeq ID
NM_019921
RefSeq Synonyms
NM_019921, BC054105,
Species
mouse
UniGene ID
Mm.274404

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.