mouse VPS4A shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Vps4a.
shAAV-276071
AAV-m-VPS4A-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Vps4a (vacuolar protein sorting 4A). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
4930589C15Rik; AI325971; AW553189
Description (Vector)
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
Gene ID
116733
Gene Name (Vector)
vacuolar protein sorting 4A
Gene Symbol
Vps4a
ORF Size (aa)
1314
ORF Size (bp)
1314 bp
RefSeq ID
NM_126165
RefSeq Synonyms
NM_126165, BC018368,
Species
mouse
UniGene ID
Mm.236004
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.