mouse TWIST2 shRNA silencing AAV

Name 
AAV-m-TWIST2-shRNA
Cat No 
shAAV-275369
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Twist2.

shAAV-275369
AAV-m-TWIST2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Twist2 (twist basic helix-loop-helix transcription factor 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
bHLHa39; Dermo1
Description (Vector)
Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism (By similarity). Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors.
Gene ID
13345
Gene Name (Vector)
twist basic helix-loop-helix transcription factor 2
Gene Symbol
Twist2
ORF Size (aa)
483
ORF Size (bp)
483 bp
RefSeq ID
NM_007855
RefSeq Synonyms
NM_007855, BC090636,
Species
mouse
UniGene ID
Mm.9474

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.