mouse TTRAP shRNA silencing AAV

Name
AAV-m-TTRAP-shRNA

Cat No
shAAV-275298

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Tdp2.

shAAV-275298

AAV-m-TTRAP-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Tdp2 (tyrosyl-DNA phosphodiesterase 2). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

D13Ertd656e; Ttrap

Description (Vector)

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.

Gene ID

56196

Gene Name (Vector)

tyrosyl-DNA phosphodiesterase 2

Gene Symbol

Tdp2

ORF Size (aa)

1113

ORF Size (bp)

1113 bp

RefSeq ID

NM_019551

RefSeq Synonyms

NM_019551, BC132513, BC132511,

Species

mouse

UniGene ID

Mm.427162

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.