mouse TRIM72 shRNA silencing AAV

Name
AAV-m-TRIM72-shRNA

Cat No
shAAV-275030

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Trim72.

shAAV-275030

AAV-m-TRIM72-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Trim72 (tripartite motif-containing 72). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

BC067209; MG53

Description (Vector)

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).

Gene ID

434246

Gene Name (Vector)

tripartite motif-containing 72

Gene Symbol

Trim72

ORF Size (aa)

1434

ORF Size (bp)

1434 bp

RefSeq ID

NM_001079932

RefSeq Synonyms

NM_001079932, BC158120, BC158111, BC147789,

Species

mouse

UniGene ID

Mm.389924

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.