mouse TFRC shRNA silencing AAV

Name
AAV-m-TFRC-shRNA

Cat No
shAAV-274094

Availability
Immediate

This is an AAV expressing shRNA for silencing of Mouse Tfrc.

shAAV-274094

AAV-m-TFRC-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Tfrc (transferrin receptor). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

2610028K12Rik; CD71; E430033M20Rik; Mtvr1; p90; TFR; TFR1; TR; Trfr

Description (Vector)

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.

Gene ID

22042

Gene Name (Vector)

transferrin receptor

Gene Symbol

Tfrc

ORF Size (aa)

2292

ORF Size (bp)

2292 bp

RefSeq ID

NM_011638

RefSeq Synonyms

NM_011638, BC054522,

Species

mouse

UniGene ID

Mm.28683

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.