mouse TEAD1 shRNA silencing AAV

Name
AAV-m-TEAD1-shRNA

Cat No
shAAV-274004

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Tead1.

shAAV-274004

AAV-m-TEAD1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Tead1 (TEA domain family member 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

2610024B07Rik; B230114H05Rik; Gtrgeo5; mTEF-1; Tcf13; TEAD-1; TEF-1

Description (Vector)

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.

Gene ID

21676

Gene Name (Vector)

TEA domain family member 1

Gene Symbol

Tead1

ORF Size (aa)

1236

RefSeq ID

NM_009346

RefSeq Synonyms

NM_009346, NM_001166585, NM_001166584, BC060138,

Species

mouse

UniGene ID

Mm.28081

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.