mouse SEMA5A shRNA silencing AAV

Name
AAV-m-SEMA5A-shRNA

Cat No
shAAV-271595

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Sema5a.

shAAV-271595

AAV-m-SEMA5A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Sema5a (sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

5930434A13; 9130201M22Rik; AI464145; Semaf; semF

Description (Vector)

SEMA5A may act as positive axonal guidance cues.

Gene ID

20356

Gene Name (Vector)

sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A

Gene Symbol

Sema5a

ORF Size (aa)

1689

ORF Size (bp)

1689 bp

RefSeq ID

NM_009154

RefSeq Synonyms

NM_009154, BC065137,

Species

mouse

UniGene ID

Mm.260374

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.