mouse RTCD1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Rtca.
shAAV-271203
AAV-m-RTCD1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Rtca (RNA 3′-terminal phosphate cyclase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
2310009A18Rik; AI450277; RPC; Rtcd1
Description (Vector)
Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
Gene ID
66368
Gene Name (Vector)
RNA 3'-terminal phosphate cyclase
Gene Symbol
Rtca
ORF Size (aa)
1101
ORF Size (bp)
1101 bp
RefSeq ID
NM_025517
RefSeq Synonyms
NM_025517, BC090403, BC016519,
Species
mouse
UniGene ID
Mm.251228
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.