mouse RAD50 shRNA silencing AAV

Name
AAV-m-RAD50-shRNA

Cat No
shAAV-270061

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Rad50.

shAAV-270061

AAV-m-RAD50-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Rad50 (RAD50 double strand break repair protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Mrell; Rad50l

Description (Vector)

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

Gene ID

19360

Gene Name (Vector)

RAD50 double strand break repair protein

Gene Symbol

Rad50

ORF Size (aa)

3939

ORF Size (bp)

3939 bp

RefSeq ID

NM_009012

RefSeq Synonyms

NM_009012, BC168398,

Species

mouse

UniGene ID

Mm.477255

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.