mouse NOTO shRNA silencing AAV

Name 
AAV-m-NOTO-shRNA
Cat No 
shAAV-265948
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Noto.

shAAV-265948
AAV-m-NOTO-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Noto (notochord homeobox). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Flh; MmNot; Not; tc
Description (Vector)
Transcription regulator acting downstream of both FOXA2 and T during notochord development. Required for node morphogenesis. Is essential for cilia formation in the posterior notochord (PNC) and for left-right patterning; acts upstream of FOXJ1 and RFX3 in this process and is required for the expression of various components important for axonemal assembly and function. Plays a role in regulating axial versus paraxial cell fate (By similarity).
Gene ID
384452
Gene Name (Vector)
notochord homeobox
Gene Symbol
Noto
ORF Size (aa)
723
RefSeq ID
NM_001007472
RefSeq Synonyms
NM_001007472, BC139155, BC139154,
Species
mouse
UniGene ID
Mm.347883

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.