mouse NENF shRNA silencing AAV

Name 
AAV-m-NENF-shRNA
Cat No 
shAAV-265662
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Nenf.

shAAV-265662
AAV-m-NENF-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Nenf (neuron derived neurotrophic factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1110060M21Rik; SCIRP10; Spuf
Description (Vector)
Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme (By similarity).
Gene ID
66208
Gene Name (Vector)
neuron derived neurotrophic factor
Gene Symbol
Nenf
ORF Size (aa)
516
ORF Size (bp)
516 bp
RefSeq ID
NM_025424
RefSeq Synonyms
NM_025424, BC048464,
Species
mouse
UniGene ID
Mm.46444

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.