mouse NEDD4 shRNA silencing AAV

Name
AAV-m-NEDD4-shRNA

Cat No
shAAV-265628

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Nedd4.

shAAV-265628

AAV-m-NEDD4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Nedd4 (neural precursor cell expressed, developmentally down-regulated 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AA959633; AL023035; AU019897; E430025J12Rik; mKIAA0093; Nedd4-1; Nedd4a

Description (Vector)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2.

Gene ID

17999

Gene Name (Vector)

neural precursor cell expressed, developmentally down-regulated 4

Gene Symbol

Nedd4

ORF Size (aa)

2664

ORF Size (bp)

2664 bp

RefSeq ID

NM_010890

RefSeq Synonyms

NM_010890, BC138813,

Species

mouse

UniGene ID

Mm.279923

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.